產(chǎn)品編號(hào) | bs-1684R-BF555 |
英文名稱(chēng) | Rabbit Anti-SKI/BF555 Conjugated antibody |
中文名稱(chēng) | BF555標(biāo)記的C-SKI抗體 |
別 名 | C-SKI; v-SKI; Sloan-kettering viral oncogene homolog; Avian sarcoma viral (v ski) oncogene homolog; C oncogene; C ski; Ski oncogene; Ski oncoprotein; SKV; v ski avian sarcoma viral oncogene homolog; v ski sarcoma viral oncogene homolog; v-ski sarcoma viral oncogene homolog; SKI_HUMAN. |
規(guī)格價(jià)格 | 100ul/2980元 購(gòu)買(mǎi) 大包裝/詢(xún)價(jià) |
說(shuō) 明 書(shū) | 100ul |
研究領(lǐng)域 | 腫瘤 轉(zhuǎn)錄調(diào)節(jié)因子 |
抗體來(lái)源 | Rabbit |
克隆類(lèi)型 | Polyclonal |
交叉反應(yīng) | (predicted: Human, Mouse, Rat, Chicken, Dog, Cow, Horse, ) |
產(chǎn)品應(yīng)用 | IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 80kDa |
性 狀 | Lyophilized or Liquid |
濃 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human C-SKI |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
儲(chǔ) 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol |
保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
產(chǎn)品介紹 |
background: SKI is a nuclear protein that forms homodimers and heterodimers and binds to DNA to function as transcriptional activators and repressors. The SKI oncoprotein dramatically affects cell growth, differentiation, and/or survival. SKI was shown to act in distinct signaling pathways including those involving nuclear receptors, transforming growth factor beta, and tumor suppressors. SKI inhibits transforming growth factor-beta (TGF-beta) signaling through interaction with Smad proteins. SKI represses Smad-mediated transcriptional activation, probably through its action as a transcriptional co-repressor. SKI also inhibits TGF-beta-induced downregulation of genes such as c-myc. Function: May play a role in terminal differentiation of skeletal muscle cells but not in the determination of cells to the myogenic lineage. Functions as a repressor of TGF-beta signaling. Subunit: Interacts with SMAD2, SMAD3 and SMAD4. Interacts with HIPK2. Part of a complex with HIPK2 and SMAD1/2/3. Interacts with PRDM16 and SMAD3; the interaction with PRDM16 promotes the recruitment SMAD3-HDAC1 complex on the promoter of TGF-beta target genes. Subcellular Location: Nucleus. Similarity: Belongs to the SKI family. Database links: Entrez Gene: 6497 Human Entrez Gene: 20481 Mouse Omim: 164780 Human SwissProt: P49140 Chicken SwissProt: P12755 Human SwissProt: Q60698 Mouse Unigene: 656507 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. SKI又稱(chēng)SKI、SKV,經(jīng)研究發(fā)現(xiàn)SKI蛋白是刺激和誘發(fā)腫瘤生長(zhǎng)導(dǎo)致癌癥的關(guān)鍵蛋白質(zhì)之一,SKI蛋白主要通過(guò)阻止TGF-β蛋白發(fā)揮作用,當(dāng)TGF-β蛋白的信號(hào)傳導(dǎo)被SKI蛋白阻斷后,細(xì)胞就可能加速增值、生長(zhǎng),最終導(dǎo)致癌癥發(fā)生。 有研究表明:c-ski可調(diào)節(jié)多種核因子的轉(zhuǎn)錄活性,參與神經(jīng)系統(tǒng)發(fā)育、造血細(xì)胞的增殖和分化、腫瘤發(fā)生、組織再生等多種生理病理過(guò)程,更詳細(xì)的作用機(jī)制還在進(jìn)一步研究之中。 |