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Annexin A2 (2F4) Recombinant Rabbit mAb (bsm-54021R)  
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產(chǎn)品編號 bsm-54021R
英文名稱 Annexin A2 (2F4) Recombinant Rabbit mAb
中文名稱 膜粘連蛋白 Ⅱ重組兔單抗
別    名 ANX 2; ANX2; ANX2L4; ANXA2; arylsulfatase B; CAL1H; Calpactin I heavy chain; calpactin I heavy polypeptide(p36); chromobindin 8; LIP2; Lipocortin II; LPC2; LPC2D; P36; P36 protein; PAP-IV; placental anticoagulant protein IV; Protein I; ANXA2_HUMAN; Annexin II; Annexin-2; Calpactin-1 heavy chain; Chromobindin-8; Placental anticoagulant protein IV.  
研究領域 腫瘤  心血管  細胞生物  信號轉(zhuǎn)導  細胞凋亡  
抗體來源 Rabbit
克隆類型 Recombinant
克 隆 號 2F4
交叉反應 Human
產(chǎn)品應用 WB=1:300-1000,IHC-P=1:100-500,IHC-F=1:200-400,IF=1:50-200,ICC/IF=1:50
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 36 kDa
檢測分子量
細胞定位 細胞漿 細胞膜 細胞外基質(zhì) 分泌型蛋白 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 Recombinant Human Annexin A2 :  1-200/339 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antib
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 This gene encodes a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption. This gene has three pseudogenes located on chromosomes 4, 9 and 10, respectively. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008].

Function:
Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response.

Subunit:
Heterotetramer containing 2 light chains of S100A10/p11 and 2 heavy chains of ANXA2/p36. Interacts with ATP1B1 and DYSF. Interacts with human cytomegalovirus (HCMV). Interacts with COCH.

Subcellular Location:
Secreted, extracellular space, extracellular matrix, basement membrane. Melanosome. Note=In the lamina beneath the plasma membrane. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Translocated from the cytoplasm to the cell surface through a Golgi-independent mechanism.

Post-translational modifications:
Phosphorylation of Tyr-24 enhances heat stress-induced translocation to the cell surface.
ISGylated.

Similarity:
Belongs to the annexin family.
Contains 4 annexin repeats.

SWISS:
P07355

Gene ID:
302

Database links:

Entrez Gene: 302 Human

Entrez Gene: 12306 Mouse

Entrez Gene: 56611 Rat

Omim: 151740 Human

SwissProt: P07355 Human

SwissProt: P07356 Mouse

SwissProt: Q07936 Rat

Unigene: 511605 Human

Unigene: 238343 Mouse

Unigene: 90546 Rat



信號傳導(Signaling Intermediates)
Annexin 2是新近發(fā)現(xiàn)的纖維蛋白溶解酶原(PLG)和組織型纖溶蛋白溶解酶原激活物(t-PA)的共受體,是Ca2+介導具有高親和力的磷脂結合蛋白.其介導血管內(nèi)外纖維蛋白溶解,對保持機體內(nèi)環(huán)境穩(wěn)定具有重要作用.
依賴性膜磷脂結合蛋白Annexin II調(diào)控異常及有害因子的作用,可導致血栓性疾病和動脈硬化癥
鈣依賴性膜磷脂結合蛋白膜粘連蛋白2異常高表達所引起纖溶亢進,是白血病等惡性腫瘤出血合并癥重要機制之一.鈣依賴性磷脂結合蛋白對其干預和調(diào)控可能為治療上述病癥提供新思路,為臨床治療開辟新途徑.
產(chǎn)品圖片
Sample: Lane 1: MCF-7 (Human) Cell Lysate at 30 ug Lane 2: Hela (Human) Cell Lysate at 30 ug Lane 3: K562 (Human) Cell Lysate at 30 ug Lane 4: A431 (Human) Cell Lysate at 30 ug Lane 5: U251 (Human) Cell Lysate at 30 ug Primary: Anti-Annexin A2 (bsm-54021R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 38 kD Observed band size: 36 kD
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