| 产品编号 | bsm-61035R |
| 英文名称 | AQP4 Recombinant Rabbit mAb |
| 中文名称 | 水通道蛋白4重组兔单抗 |
| 别 名 | aquaporin 4; WCH4; Mercurial-insensitive water channel; MIWC; AQP 4; AQP4; Aquaporin4; HMIWC 2; HMIWC2; Mercurial insensitive water channel; MGC22454; MIWC; AQP4_HUMAN; Aquaporin-4; AQP-4. |
| 抗体来源 | Rabbit |
| 克隆类型 | Recombinant |
| 克 隆 号 | 9F10 |
| 交叉反应 | Human,Mouse,Rat |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 36 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human Aquaporin 4: 300-323 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a member of the aquaporin family of intrinsic membrane proteins that function as water-selective channels in the plasma membranes of many cells. The encoded protein is the predominant aquaporin found in brain. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Function: Forms a water-specific channel. Osmoreceptor which regulates body water balance and mediates water flow within the central nervous system. Subunit: Homotetramer. Part of a complex containing MLC1, TRPV4, HEPACAM and ATP1B1. Subcellular Location: Membrane; Multi-pass membrane protein. Tissue Specificity: Brain, muscle, heart, kidney, lung, and trachea. Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family. SWISS: P55087 Gene ID: 361 Database links: Entrez Gene: 361 Human Entrez Gene: 11829 Mouse Omim: 600308 Human SwissProt: P55087 Human SwissProt: P55088 Mouse Unigene: 281008 Cow Unigene: 315369 Human Unigene: 250786 Mouse Unigene: 90091 Rat 通道蛋白（Channel Protein） 水通道蛋白(AQPs)广泛分布于机体不同组织器官中,脑组织中的水通道蛋白主要为AQP4. AQP-4蛋白主要在星型胶质细胞和室管膜细胞内表达,尤其在于毛细血管和软脑膜直接接触的胶质细胞上及血管周表达丰富. 水通道蛋白4的分布特点与其功能密切相关,可能是脑脊液重吸收、渗透压调节、脑水肿形成等生理、病理过程的分子生物学基础.研究水通道蛋白4可为脑水肿及水代谢疾病提供分子水平的理论依据,同时为临床治疗脑水肿及水代谢疾病提供一种新途径. |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AQP4 Monoclonal Antibody, Unconjugated(bsm-61035R) at 1:100 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AQP4 Monoclonal Antibody, Unconjugated(bsm-61035R) at 1:100 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AQP4 Monoclonal Antibody, Unconjugated(bsm-61035R) at 1:100 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AQP4 Monoclonal Antibody, Unconjugated(bsm-61035R) at 1:100 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AQP4 Monoclonal Antibody, Unconjugated(bsm-61035R) at 1:100 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AQP4 Monoclonal Antibody, Unconjugated(bsm-61035R) at 1:100 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with AQP4 Monoclonal Antibody, Unconjugated(bsm-61035R) at 1:100 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
