| 產(chǎn)品編號(hào) | bsm-60464M |
| 英文名稱 | MTHFD1 Recombinant Mouse mAb |
| 中文名稱 | 亞甲基四氫葉酸脫氫酶單克隆抗體 |
| 別 名 | C1TC_HUMAN; C-1-tetrahydrofolate synthase, cytoplasmic; MTHFC; MTHFD; C1-THF synthase; Epididymis secretory sperm binding protein; C-1-tetrahydrofolate synthase, cytoplasmic, N-terminally processed; Methylenetetrahydrofolate dehydrogenase; methylenetetrahydrofolate dehydrogenase, cyclohydrolase and formyltetrahydrofolate synthetase 1; CIMAH; |
| 研究領(lǐng)域 | 腫瘤 細(xì)胞生物 信號(hào)轉(zhuǎn)導(dǎo) 新陳代謝 |
| 抗體來(lái)源 | Mouse |
| 克隆類型 | Recombinant |
| 克 隆 號(hào) | F9D9 |
| 交叉反應(yīng) | Human (predicted: Mouse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-1:1000,ICC/IF=1:50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 110 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 亞 型 | IgG1, Kappa |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | PBS, Glycerol, BSA. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a protein that possesses three distinct enzymatic activities, 5,10-methylenetetrahydrofolate dehydrogenase, 5,10-methenyltetrahydrofolate cyclohydrolase and 10-formyltetrahydrofolate synthetase. Each of these activities catalyzes one of three sequential reactions in the interconversion of 1-carbon derivatives of tetrahydrofolate, which are substrates for methionine, thymidylate, and de novo purine syntheses. The trifunctional enzymatic activities are conferred by two major domains, an aminoterminal portion containing the dehydrogenase and cyclohydrolase activities and a larger synthetase domain. [provided by RefSeq, Jul 2008] Subcellular Location: Cytoplasm. Tissue Specificity: Ubiquitous. Database links: Entrez Gene: 4522 Human Entrez Gene: 108156 Mouse Omim: 172460 Human SwissProt: P11586 Human SwissProt: Q922D8 Mouse |
| 產(chǎn)品圖片 |
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: room
temperature 2h
Secondary Ab: Goat Anti-Mouse IgG H&L
(HRP)
Lysate: 1: HeLa, 2: 293, 3: A20, 4: Raw264.7
Protein loading quantity: 20 μg
Exposure time: 10 s
Predicted MW: 102 kDa
Observed MW: 102 kDa
Cell line: HEK-293
Fixative: 100% Ice-cold methanol
Permeabilization: 0.1% TritonX-100
Primary Ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary Ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (Blue)
Comment: Color green is the positive signal for bsm-60464M
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