| 產(chǎn)品編號 | bs-42001R |
| 英文名稱 | GFAP Rabbit pAb |
| 中文名稱 | 膠質(zhì)纖維酸性蛋白抗體 |
| 別 名 | Astrocyte Marker; FLJ45472; GFAP; Glial Fibrillary Acidic Protein; Intermediate filament protein; GFAP_HUMAN. |
| 研究領域 | 腫瘤 細胞生物 免疫學 神經(jīng)生物學 信號轉(zhuǎn)導 干細胞 細胞粘附分子 細胞類型標志物 細胞骨架 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Human |
| 產(chǎn)品應用 | WB=1:5000-20000,ICC/IF=1:50-200,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 48 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant human GFAP protein: 1-390/432 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008] SWISS: P14136 Gene ID: 2670 Database links:
Entrez Gene: 2670 Human Entrez Gene: 14580 Mouse Omim: 137780 Human SwissProt: P14136 Human SwissProt: P03995 Mouse
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| 產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with GFAP polyclonal antibody, unconjugated (bs-42001R) at 1:20000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
4% Paraformaldehyde-fixed U87-MG (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (GFAP) polyclonal Antibody, unconjugated (bs-42001R) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-40295G-FITC) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
Measured by its binding ability in a indirect ELISA. Immobilized Human GFAP protein, His Tag (Cat. bs-42001P) at 2 μg/mL (100 μL/well) can bind ?Rabbit Anti-Human GFAP Antibody, the EC50 is 540.1 ng/mL.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
