| 產(chǎn)品編號(hào) | bsm-60298R |
| 英文名稱(chēng) | CD134 Recombinant Rabbit mAb |
| 中文名稱(chēng) | CD134重組兔單抗 |
| 別 名 | CD134/OX40L receptor; Tumor necrosis factor receptor superfamily member 4; TNFRSF4; ACT 35; ACT35; ACT35 antigen; CD 134; CD134 antigen; Lymphoid activation antigene ACT35; OX 40; OX40; OX40 antigen; OX40 cell surface antigen; OX40 homologue; OX40L receptor; TAX transcriptionally activated glycoprotein 1 receptor; TNFRSF 4; TNR4_HUMAN. |
| 研究領(lǐng)域 | 免疫學(xué) 干細(xì)胞 |
| 抗體來(lái)源 | Rabbit |
| 克隆類(lèi)型 | Recombinant |
| 克 隆 號(hào) | 2H6 |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:50-200,IHC-F=1:50-200,IF=1:50-200,Flow-Cyt=1:50-100,ICC/IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 27 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | A synthesized peptide derived from human TNFRSF4: 1-277/277 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor has been shown to activate NF-kappaB through its interaction with adaptor proteins TRAF2 and TRAF5. Knockout studies in mice suggested that this receptor promotes the expression of apoptosis inhibitors BCL2 and BCL2lL1/BCL2-XL, and thus suppresses apoptosis. The knockout studies also suggested the roles of this receptor in CD4+ T cell response, as well as in T cell-dependent B cell proliferation and differentiation. [provided by RefSeq, Jul 2008]. SWISS: P43489 Gene ID: 7293 Database links: Entrez Gene: 7293 Human SwissProt: P43489 Human |
| 產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with CD134 monoclonal antibody, unconjugated (bsm-60298R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD134 Monoclonal Antibody, Unconjugated (bsm-60298R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD134 Monoclonal Antibody, Unconjugated (bsm-60298R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD134 Monoclonal Antibody, Unconjugated (bsm-60298R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
4% Paraformaldehyde-fixed MJ (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (CD134) monoclonal Antibody, unconjugated (bsm-60298R) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
The MJ (H) cells were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.),followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-CD134 antibody (bsm-60298R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
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