| 產品編號 | bsm-51247M |
| 英文名稱 | FABP4 Mouse mAb |
| 中文名稱 | 脂肪細胞型脂肪酸結合蛋白單克隆抗體 |
| 別 名 | adipocyte; 3T3-L1 lipid-binding protein; 422/aP2; A FABP; A-FABP; Adipocyte lipid binding protein; Adipocyte lipid-binding protein; Adipocyte protein AP2; Adipocyte-type fatty acid-binding protein; AFABP; ALBPv ALBP/Ap2; aP2; FABP; FABP4_HUMAN; Fatty acid binding protein 4 adipocyte; Fatty acid binding protein 4; Fatty acid binding protein adipocyte; Fatty acid-binding protein 4; Fatty acid-binding protein; Lbpl; Myelin P2 protein homolog; P15v P2 adipocyte protein; Protein 422. |
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Specific References (5) | bsm-51247M has been referenced in 5 publications.
[IF=7.012] Mengqi Su. et al. Epididymal white adipose tissue promotes angiotensin II-induced cardiac fibrosis in an exosome-dependent manner. TRANSL RES. 2022 May;: FC ; Mouse.
[IF=5.923] Yonglin Hua. et al. Ablation of KDM2A Inhibits Preadipocyte Proliferation and Promotes Adipogenic Differentiation. Int J Mol Sci. 2021 Jan;22(18):9759 IF ; Mouse.
[IF=5.201] Xuemei Shen. et al. CircINSR Regulates Fetal Bovine Muscle and Fat Development. Front Cell Dev Biol. 2020; 8: 615638 WB ; Bovine.
[IF=4.861] Xuemei Shen. et al. CircRNF111 Contributes to Adipocyte Differentiation by Elevating PPARγ Expression via miR-27a-3p. EPIGENETICS-US. 2022 Nov 15 WB ; Bovine.
[IF=1.966] Zhang Y et al. High expression of FABP4 and FABP6 in patients with colorectal cancer. World J Surg Oncol. 2019 Oct 24;17(1):171. IHC-P&WB ; Human.
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| 研究領域 | 腫瘤 免疫學 轉錄調節(jié)因子 |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號 | 6F3 |
| 交叉反應 | Human,Mouse |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 14 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant human FABP4 protein |
| 亞 型 | |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
FABP4 encodes the fatty acid binding protein found in adipocytes. Fatty acid binding proteins are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. It is thought that FABPs roles include fatty acid uptake, transport, and metabolism. [provided by RefSeq]. Function: Lipid transport protein in adipocytes. Binds both long chain fatty acids and retinoic acid. Delivers long-chain fatty acids and retinoic acid to their cognate receptors in the nucleus. Subunit: Monomer. Homodimer. Interacts with PPARG. Subcellular Location: Cytoplasm. Nucleus. Note=Depending on the nature of the ligand, a conformation change exposes a nuclear localization motif and the protein is transported into the nucleus. Subject to constitutive nuclear export. Similarity: Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family. SWISS: P15090 Gene ID: 2167 Database links: Entrez Gene: 2167 Human Entrez Gene: 11770 Mouse Omim: 600434 Human SwissProt: P15090 Human SwissProt: P04117 Mouse Unigene: 391561 Human Unigene: 582 Mouse Unigene: 4258 Rat |
| 產品圖片 |
Sample:
Lane 1: Mouse Adipose tissue lysates
Lane 2: Mouse Breast tissue lysates
Lane 3: Mouse Heart tissue lysates
Lane 4: Mouse Thymus tissue lysates
Primary: Anti-FABP4 (bsm-51247M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 14 kDa
Observed band size: 14 kDa
Paraformaldehyde-fixed, paraffin embedded Human Breast; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with FABP4 Monoclonal Antibody, Unconjugated (bsm-51247M) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Ovarian Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with FABP4 Monoclonal Antibody, Unconjugated (bsm-51247M) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Brown Fat; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with FABP4 Monoclonal Antibody, Unconjugated (bsm-51247M) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with FABP4 Monoclonal Antibody, Unconjugated (bsm-51247M) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
