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pan-cytokeratin Rabbit pAb (bs-5352R)  
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產(chǎn)品編號 bs-5352R
英文名稱 pan-cytokeratin Rabbit pAb
中文名稱 廣譜細(xì)胞角蛋白PCK抗體
別    名 P-CK; pan-cytokeratin; pan-CK; pan CK; CK-PAN; wide spectrum Cytokeratin; Cytokeratins; [CK4; CK75; CK3; CK2; CK6C; CK6A; keratin 6E].  
研究領(lǐng)域 腫瘤  信號轉(zhuǎn)導(dǎo)  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應(yīng) Human,Mouse,Rat (predicted: Rabbit,Pig,Cow,Dog,Horse)
產(chǎn)品應(yīng)用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 kDa
檢測分子量
細(xì)胞定位 細(xì)胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human pan-cytokeratin: 331-430/590 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 Preservative: 0.02% Proclin300, Constituents: 1% BSA, 0.01M PBS, pH7.4.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 Cytokeratins are proteins of keratin-containing intermediate filaments found in the intracytoplasmic cytoskeleton of epithelial tissue. The cytokeratins are encoded by a family encompassing 30 genes. Among them, 20 are epithelial genes and the remaining 10 are specific for trichocytes. In the cytoplasm, the keratin filaments conform a complex network which extends from the surface of the nucleus to the cell membrane. Numerous accessory proteins are involved in the genesis and maintenance of such structure. This association between the plasma membrane and the nuclear surface provides important implications for the organization of the cytoplasm and cellular communication mechanisms. Apart from the relatively static functions provided in terms of supporting the nucleus and providing tensile strength to the cell, the cytokeratin networks undergo rapid phosphate exchanges mediated depolymerization, with important implications in the more dynamic cellular processes such as mitosis and post-mitotic period, cell movement and differentiation. Cytokeratins interact with desmosomes and hemidesmosomes, thus collaborating to cell-cell adhesion and basal cell-underlying connective tissue connection.

Subcellular Location:
Cytoplasmic.

Tissue Specificity:
epithelial cells

SWISS:
Q2M2I5

Gene ID:
192666

產(chǎn)品圖片
Sample: Lane 1: Skin (Mouse) Lysate at 40 ug Lane 2: Uterus (Mouse) Lysate at 40 ug Lane 3: Urinary bladder (Mouse) Lysate at 40 ug Lane 4: Esophagus (Mouse) Lysate at 40 ug Lane 5: Skin (Rat) Lysate at 40 ug Lane 6: Uterus (Rat) Lysate at 40 ug Lane 7: Urinary bladder (Rat) Lysate at 40 ug Lane 8: Esophagus (Rat) Lysate at 40 ug Primary: Anti-Cytokeratin 5 (bs-5352R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 62 kD Observed band size: 60 kD
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