| 產品編號 | bs-21072R |
| 英文名稱 | p66 alpha/GATAD2A Rabbit pAb |
| 中文名稱 | p66 α蛋白抗體 |
| 別 名 | FLJ20085; FLJ21017; GATA zinc finger domain containing 2A; GATA zinc finger domain containing protein 2A; GATA zinc finger domain-containing protein 2A; GATAD2A; Hp66 alpha; Hp66alpha; OTTHUMP00000201911; OTTHUMP00000201912; P66 alpha; P66A_HUMAN; p66alpha; Transcriptional repressor p66 alpha; Transcriptional repressor p66-alpha; Transcriptional repressor p66alpha. |
| 研究領域 | 細胞生物 神經(jīng)生物學 轉錄調節(jié)因子 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Mouse (predicted: Human) |
| 產品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 68 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human p66 alpha: 151-250/633 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
GATAD2A (GATA Zinc Finger Domain Containing 2A) is a Protein Coding gene. Among its related pathways are Activated PKN1 stimulates transcription of AR (androgen receptor) regulated genes KLK2 and KLK3 and Development NOTCH1-mediated pathway for NF-KB activity modulation. GO annotations related to this gene include transcription factor activity, sequence-specific DNA binding and protein binding, bridging. An important paralog of this gene is GATAD2B. Function: Transcriptional repressor. Subcellular Location: Nucleus speckle. Tissue Specificity: Ubiquitous, both in fetal and adult tissues. Similarity: Contains 1 GATA-type zinc finger. SWISS: Q86YP4 Gene ID: 54815 Database links: Entrez Gene: 54815 Human Entrez Gene: 234366 Mouse SwissProt: Q86YP4 Human SwissProt: Q8CHY6 Mouse Unigene: 118964 Human |
| 產品圖片 |
Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (p66 alpha) Polyclonal Antibody, Unconjugated (bs-21072R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
