| 產(chǎn)品編號(hào) | bs-18203R |
| 英文名稱 | LDB2 Rabbit pAb |
| 中文名稱 | LDB2蛋白抗體 |
| 別 名 | Carboxyl terminal LIM domain binding protein 1; Carboxyl terminal LIM domain protein 1; Carboxyl-terminal LIM domain-binding protein 1; CLIM 1; CLIM-1; CLIM1; LDB 1; LDB 2; LDB-2; LDB1; LDB2; LDB2_HUMAN; LIM binding domain 2; LIM domain binding 2; LIM domain binding factor 2; LIM domain binding factor CLIM 1; LIM domain binding factor CLIM1; LIM domain binding protein 2; LIM domain-binding factor CLIM1; LIM domain-binding protein 2; OTTHUMP00000158632; OTTHUMP00000217823; OTTHUMP00000217824; OTTHUMP00000217825; OTTHUMP00000217827. |
| 研究領(lǐng)域 | 細(xì)胞生物 免疫學(xué) 轉(zhuǎn)錄調(diào)節(jié)因子 表觀遺傳學(xué) |
| 抗體來(lái)源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號(hào) | |
| 交叉反應(yīng) | Human (predicted: Mouse,Rat,Rabbit,Cow,Dog,Cat,Horse) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 43 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human LDB2: 1-100/373 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Genes encoding LIM domain-binding factors were initially isolated in a screen for proteins that physically interact with the LIM domains of nuclear proteins (summarized by Semina et al., 1998 [PubMed 9799849]). These proteins, such as the one encoded by the LDB2 gene, are capable of binding to a variety of transcription factors and are likely to function at enhancers to bring together diverse transcription factors and form higher order activation complexes or to block formation of such complexes (Jurata and Gill, 1997 [PubMed 9315627]). The family of genes encoding LIM domain-binding factors includes 2 members isolated from the mouse, Clim1 (Bach et al., 1997 [PubMed 9192866]) and Clim2/Lbd1/Nli (Agulnick et al., 1996 [PubMed 8918878]; Jurata et al., 1996 [PubMed 8876198]; Bach et al., 1997 [PubMed 9192866]) and their homologs cloned from the frog, chicken, and fly. The fact that LIM domain-binding factors are likely to be involved in the coordination of the transcriptional activity of many diverse factors might implicate them in human phenotypes characterized by multiple affected sites.[supplied by OMIM, Jul 2010] Function: Binds to the LIM domain of a wide variety of LIM domain-containing transcription factors. Subcellular Location: Nucleus. Post-translational modifications: Ubiquitinated by RLIM/RNF12, leading to its degradation by the proteasome. Similarity: Belongs to the LDB family. SWISS: O43679 Gene ID: 9079 Database links: Entrez Gene: 9079 Human Entrez Gene: 16826 Mouse Omim: 603450 Human SwissProt: Q9W676 Chicken SwissProt: O43679 Human SwissProt: O55203 Mouse Unigene: 714330 Human Unigene: 25785 Mouse |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (LDB2) Polyclonal Antibody, Unconjugated (bs-18203R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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