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HIF Prolyl Hydroxylases Rabbit pAb (bs-15487R)  
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產(chǎn)品編號(hào) bs-15487R
英文名稱 HIF Prolyl Hydroxylases Rabbit pAb
中文名稱 缺氧誘導(dǎo)因子脯氨酰羥化酶4抗體
別    名 PHD4/prolyl hydroxylase; EGLN4; FLJ20262; HIF prolyl hydroxylase PH4; HIF-PH4; HIF-prolyl hydroxylase 4; HIFPH4; HPH-4; Hypoxia inducible factor prolyl 4 hydroxylase; Hypoxia inducible factor prolyl hydroxylase; Hypoxia-inducible factor prolyl hydroxylase 4; P4H TM; P4H with transmembrane domain; P4H-TM; P4htm; P4HTM_HUMAN; PH 4; PH4; PHD4; Proline 4 hydroxylase; Prolyl 4 hydroxylase transmembrane(endoplasmic reticulum); Prolyl hydroxlase domain containing 4; Transmembrane prolyl 4-hydroxylase.  
研究領(lǐng)域 心血管  細(xì)胞生物  免疫學(xué)  染色質(zhì)和核信號(hào)  表觀遺傳學(xué)  
抗體來(lái)源 Rabbit
克隆類型 Polyclonal
克 隆 號(hào)
交叉反應(yīng) Mouse,Rat (predicted: Human,Pig)
產(chǎn)品應(yīng)用 WB=1:500-2000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 57 kDa
檢測(cè)分子量
細(xì)胞定位 細(xì)胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human HIF Prolyl Hydroxylases: 201-300/502 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 The product of this gene belongs to the family of prolyl 4-hydroxylases. This protein is a prolyl hydroxylase that may be involved in the degradation of hypoxia-inducible transcription factors under normoxia. It plays a role in adaptation to hypoxia and may be related to cellular oxygen sensing. Alternatively spliced variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Function:
Catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF1A at 'Pro-402' and 'Pro-564'. May function as a cellular oxygen sensor and, under normoxic conditions, may target HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex.

Subunit:
Homodimer.

Subcellular Location:
Endoplasmic reticulum membrane; Single-pass type II membrane protein.

Tissue Specificity:
Widely expressed with highest levels in adult pancreas, heart, skeletal muscle, brain, placenta, kidney and adrenal gland. Expressed at lower levels in epiphyseal cartilage and in fibroblasts.

Post-translational modifications:
Glycosylated.

Similarity:
Contains 2 EF-hand domains.
Contains 1 Fe2OG dioxygenase domain.

SWISS:
Q9NXG6

Gene ID:
54681

Database links:

Entrez Gene: 54681 Human

SwissProt: Q9NXG6 Human

Unigene: 654944 Human



產(chǎn)品圖片
Sample: Lane 1: Mouse Cerebrum tissue lysates Lane 2: Mouse Placenta tissue lysates Lane 3: Rat Cerebrum tissue lysates Primary: Anti-HIF Prolyl Hydroxylases (bs-15487R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 57 kDa Observed band size: 52 kDa
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