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HELZ Rabbit pAb (bs-15450R)  
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產品編號 bs-15450R
英文名稱 HELZ Rabbit pAb
中文名稱 人腫瘤抑制蛋白抗體
別    名 DKFZp586G1924; Down regulated in human cancers; DRHC; Helicase with zinc finger; HUMORF 5; HUMORF5; KIAA0054; MGC163454; Probable helicase with zinc finger domain; HELZ_HUMAN.  
研究領域 細胞生物  免疫學  染色質和核信號  表觀遺傳學  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應 Rat (predicted: Human,Mouse)
產品應用 IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1μg/Test
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 219 kDa
檢測分子量
細胞定位 細胞核 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human HELZ: 1-100/1942 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 Helicases comprise a class of enzymes that function as motor proteins which move along nucleic acid phosphodiester bonds, effectively separating two annealed nucleic acid strands. RNA helicases alter the conformation of RNA, specifically by unwinding double-stranded RNA regions to yield single RNA strands, a process which changes the biological activity of the RNA molecule. HELZ (helicase with zinc finger), also known as DHRC or HUMORF5, is a 1,942 amino acid nuclear protein that contains one C3H1-type zinc finger and belongs to the RNA helicase superfamily. Expressed ubiquitously during embryonic development, HELZ is thought to function as an RNA helicase that modifies RNA structure and plays a role in the development of multiple organs and tissues within the developing embryo.

Function:
May act as a helicase that plays a role in RNA metabolism in multiple tissues and organs within the developing embryo.

Subunit:
Interacts with SMYD2 (By similarity). Interacts with POLR2A. Interacts with SMYD3; the interaction may bridge SMYD3 and RNA polymerase II.

Subcellular Location:
Nucleus (Probable).

Tissue Specificity:
Expressed predominantly in thymus and brain. Expression is down-regulated in 28 of 95 tested cancer cell lines.

Similarity:
Belongs to the DNA2/NAM7 helicase family.
Contains 1 C3H1-type zinc finger.

SWISS:
P42694

Gene ID:
9931

Database links:

Entrez Gene: 9931 Human

Entrez Gene: 78455 Mouse

Entrez Gene: 287773 Rat

Omim: 606699 Human

SwissProt: P42694 Human

SwissProt: Q6DFV5 Mouse



產品圖片
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-HELZ Polyclonal Antibody, Unconjugated(bs-15450R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control: RSC96(blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice.. Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1μg in 100 μL1X PBS containing 0.5% BSA(green).
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