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EIF5 Rabbit pAb (bs-10035R)  
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50ul/1180.00元
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產(chǎn)品編號 bs-10035R
英文名稱 EIF5 Rabbit pAb
中文名稱 真核翻譯起始因子5抗體
別    名 EIF 5; EIF 5A; Eukaryotic initiation factor 5; Eukaryotic translation initiation factor 5; IF5_HUMAN.  
研究領(lǐng)域 細(xì)胞生物  免疫學(xué)  染色質(zhì)和核信號  表觀遺傳學(xué)  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應(yīng) Mouse,Rat (predicted: Human,Rabbit,Cow,Horse)
產(chǎn)品應(yīng)用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 47 kDa
檢測分子量
細(xì)胞定位 細(xì)胞漿 細(xì)胞膜 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human EIF5: 331-431/431 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 EIF5, Eukaryotic initiation factor 5, catalyzes the hydrolysis of GTP bound to the 40S ribosomal initiation complex with the subsequent joining of a 60S ribosomal subunit resulting in the release of eIF2 and the guanine nucleotide. The subsequent joining of a 60S ribosomal subunit results in the formation of a functional 80S initiation complex.

Function:
Catalyzes the hydrolysis of GTP bound to the 40S ribosomal initiation complex (40S.mRNA.Met-tRNA[F].eIF-2.GTP) with the subsequent joining of a 60S ribosomal subunit resulting in the release of eIF-2 and the guanine nucleotide. The subsequent joining of a 60S ribosomal subunit results in the formation of a functional 80S initiation complex (80S.mRNA.Met-tRNA[F]).

Similarity:
Belongs to the eIF-2-beta/eIF-5 family.
Contains 1 W2 domain.

SWISS:
P55010

Gene ID:
1983

Database links:

Entrez Gene: 1983 Human

Entrez Gene: 100047658 Mouse

Entrez Gene: 217869 Mouse

Entrez Gene: 56783 Rat

Omim: 601710 Human

SwissProt: P55010 Human

SwissProt: P59325 Mouse

SwissProt: Q07205 Rat

Unigene: 433702 Human

Unigene: 271222 Mouse

Unigene: 40123 Rat



產(chǎn)品圖片
25 ug total protein per lane of various lysates (see on figure) probed with EIF5 polyclonal antibody, unconjugated (bs-10035R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded(rat pancreas); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (EIF5) Polyclonal Antibody, Unconjugated (bs-10035R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse pancreas); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (EIF5) Polyclonal Antibody, Unconjugated (bs-10035R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
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