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Phospho-Dab1 (Tyr198) Rabbit pAb (bs-11548R)  
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產(chǎn)品編號(hào) bs-11548R
英文名稱 Phospho-Dab1 (Tyr198) Rabbit pAb
中文名稱 磷酸化磷脂蛋白受體Dab1抗體
別    名 Dab1(phospho Y198); Dab1(phospho-Tyr198); Dab 1; Dab1; Dab-1; DAB1_HUMAN; Disabled homolog 1; Disabled homolog 1 Drosophila; Scm; Scr; Scrambler; Yot; Yotari.  
產(chǎn)品類型 磷酸化抗體 
研究領(lǐng)域 細(xì)胞生物  神經(jīng)生物學(xué)  信號(hào)轉(zhuǎn)導(dǎo)  細(xì)胞膜受體  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號(hào)
交叉反應(yīng) Human,Rat (predicted: Mouse,Rabbit,Pig,Cow,Chicken,Dog,Horse)
產(chǎn)品應(yīng)用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 65 kDa
檢測(cè)分子量
細(xì)胞定位 細(xì)胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthesised phosphopeptide derived from human Dab1 around the phosphorylation site of Tyr198: V(p-Y)QY 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 The laminar organization of multiple neuronal types in the cerebral cortex is required for normal cognitive function. In mice, the disabled-1 gene plays a central role in brain development, directing the migration of cortical neurons past previously formed neurons to reach their proper layer. This gene is similar to disabled-1, and the protein encoded by this gene is thought to be a signal transducer that interacts with protein kinase pathways to regulate neuronal positioning in the developing brain. Alternatively spliced transcript variants of this gene have been reported, but their full length nature has not been determined. [provided by RefSeq, Jul 2008]

Function:
Adapter molecule functioning in neural development. May regulate SIAH1 activity.

Subunit:
Associates with the SH2 domains of SRC, FYN and ABL. Interacts with DAB2IP and SIAH1. Interacts with LRP1.

Post-translational modifications:
Phosphorylated on Tyr-198 and Tyr-220 upon reelin induction in embryonic neurons. Also phosphorylated on Ser-524 independently of reelin signaling.

Similarity:
Contains 1 PID domain.

SWISS:
O75553

Gene ID:
1600

Database links:

Entrez Gene: 1600 Human

Entrez Gene: 13131 Mouse

Entrez Gene: 266729 Rat

Entrez Gene: 374083 Chicken

Omim: 603448 Human

SwissProt: O75553 Human

SwissProt: P97318 Mouse

SwissProt: Q8CJH2 Rat

Unigene: 477370 Human

Unigene: 289682 Mouse

Unigene: 206534 Rat




產(chǎn)品圖片
Sample: Lane 1: Human HeLa cell lysates Lane 2: Human HepG2 cell lysates Lane 3: Human MCF-7 cell lysates Lane 4: Human SH-SY5Y cell lysates Primary: Anti-Phospho-Dab1 (Tyr198) (bs-11548R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 65 kDa Observed band size: 68 kDa
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Phospho-Dab1 (Tyr198) Polyclonal Antibody, Unconjugated(bs-11548R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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