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PP4R3B Rabbit pAb (bs-8328R)  
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50ul/1180.00元
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產(chǎn)品編號 bs-8328R
英文名稱 PP4R3B Rabbit pAb
中文名稱 絲氨酸/蘇氨酸蛋白磷酸酶3B抗體
別    名 Protein Phosphatase 4, Regulatory Subunit 3 beta; smk1; FLFL2; PPP4R3B; PSY2; Serine/threonine-protein phosphatase 4 regulatory subunit 3B; SMEK 2; SMEK homolog 2; SMEK homolog 2 suppressor of mek1(Dictyostelium); SMEK homolog 2 suppressor of mek1; FLJ31474; KIAA1387; PP4R3 beta; PP4R3B; PPP4R3 beta; P4R3B_HUMAN.  
研究領(lǐng)域 腫瘤  細胞生物  免疫學  發(fā)育生物學  信號轉(zhuǎn)導  表觀遺傳學  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應 Rat (predicted: Human,Mouse,Rabbit,Pig,Sheep,Cow,Chicken,Dog,Horse)
產(chǎn)品應用 IHC-P=1:100-500,IHC-F=1:100-500,IF=1:50-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 97 kDa
檢測分子量
細胞定位 細胞核 細胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human SMEK2/PP4R3B: 701-800/849 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 SMEK2 contains a WH1 domain. There are five named isoforms produced by alternative splicing. Mapping of a diet-induced hypercholesterolemia locus (Dihc2) in rats identified SMEK2 as a strong candidate for responsiveness to dietary cholesterol.

Function:
Regulatory subunit of serine/threonine-protein phosphatase 4 (PP4). May regulate the activity of PPP4C at centrosomal microtubule organizing centers.

Subunit:
Serine/threonine-protein phosphatase 4 (PP4) occurs in different assemblies of the catalytic and one or more regulatory subunits. Component of the PP4 complex PPP4C-PPP4R2-PPP4R3B.

Subcellular Location:
Cytoplasm. Cytoskeleton. Centrosome. Nucleus. Note: In interphase localized in the cytoplasm and (with higher levels) the nucleus. During metaphase located in pericentriolar regions.

Tissue Specificity:
Moderately expressed in tissues and specific brain regions examined.

Similarity:
Belongs to the SMEK family.
Contains 1 WH1 domain.

SWISS:
Q5MIZ7

Gene ID:
57223

Database links:

Entrez Gene: 57223 Human

Entrez Gene: 104570 Mouse

Omim: 610352 Human

SwissProt: Q5MIZ7 Human

SwissProt: Q922R5 Mouse

Unigene: 516182 Human

Unigene: 276386 Mouse



產(chǎn)品圖片
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PP4R3B) Polyclonal Antibody, Unconjugated (bs-8328R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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