| 產(chǎn)品編號(hào) | bs-6864R |
| 英文名稱 | NALP12 Rabbit pAb |
| 中文名稱 | NALP12抗體 |
| 別 名 | CLR19.3; FCAS2; Monarch 1; Monarch-1; NACHT, leucine rich repeat and PYD containing 12; NACHT, LRR and PYD containing protein 12; NACHT, LRR and PYD domains-containing protein 12; NAL12_HUMAN; NLR family, pyrin domain containing 12; NLRP12; Nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domain containing 12; PAN6; PYPAF7; PYRIN containing APAF1 like protein 7; PYRIN-containing APAF1-like protein 7; Regulated by nitric oxide; RNO; RNO2. |
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Specific References (1) | bs-6864R has been referenced in 1 publications.
[IF=5.714] Xue-ni Niu. et al. 7-O-(2- (Propylamino)-2-oxoethyl) hesperetin attenuates inflammation and protects against alcoholic liver injury by NLRP12. INT IMMUNOPHARMACOL. 2022 Sep;110:109006 IF, WB ; Mouse.
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| 研究領(lǐng)域 | 細(xì)胞生物 信號(hào)轉(zhuǎn)導(dǎo) 細(xì)胞凋亡 轉(zhuǎn)錄調(diào)節(jié)因子 |
| 抗體來(lái)源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號(hào) | |
| 交叉反應(yīng) | Human,Rat (predicted: Mouse,Rabbit,Pig,Sheep,Cow,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 117 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NALP12: 176-270/1061 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
May mediate activation of CASP1 via ASC and promote activation of NF-kappa-B via IKK.NALP proteins are cytoplasmic proteins that form a subfamily within the larger CATERPILLER family and are thought to play a crucial role in cell proliferation and reproduction. Like all other NALP family members, NALP12, also known as Monarch-1, has a C-terminal leucine-rich repeat (LRR) region, an N-terminal Pyrin domain (PYD) followed by a NACHT domain, and a NACHT-associated domain. NALP12 is thought to act as an attenuating factor of inflammation by suppressing inflammatory responses such as NF-kB activation by TLR-signaling molecules MyD88, IRAK-1, TRAF6 and RIPK1 in activated monocytes. Recent evidence suggests that mutations in NALP12 result in hereditary periodic fever syndromes. Function: May mediate activation of CASP1 via ASC and promote activation of NF-kappa-B via IKK. Subunit: Binds to ASC with its DAPIN domain. Interacts with FAF1 UBA domain via its DAPIN domain. Subcellular Location: Cytoplasm. Tissue Specificity: Detected only in peripheral blood leukocytes, predominantly in eosinophils and granulocytes, and at lower levels in monocytes. DISEASE: Defects in NLRP12 are the cause of familial cold autoinflammatory syndrome type 2 (FCAS2) [MIM:611762]. FCAS are rare autosomal dominant systemic inflammatory diseases characterized by episodes of rash, arthralgia, fever and conjunctivitis after generalized exposure to cold. Similarity: Belongs to the NLRP family. Contains 1 DAPIN domain. Contains 8 LRR (leucine-rich) repeats. Contains 1 NACHT domain. SWISS: P59046 Gene ID: 91662 Database links: Entrez Gene: 91662 Human Entrez Gene: 378425 Mouse Omim: 609648 Human SwissProt: P59046 Human SwissProt: Q08EE9 Mouse SwissProt: Q14BA2 Mouse Unigene: 631573 Human |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NALP12) Polyclonal Antibody, Unconjugated (bs-6864R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-NALP12 Polyclonal Antibody, Unconjugated(bs-6864R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: Human lung cancer issue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-NALP12 Polyclonal Antibody, Unconjugated(bs-6864R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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