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Phospho-FRA1 (Ser265) Rabbit pAb (bs-3158R)  
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產(chǎn)品編號(hào) bs-3158R
英文名稱 Phospho-FRA1 (Ser265) Rabbit pAb
中文名稱 磷酸化FRA-1蛋白抗體
別    名 FOSL1; Fos related antigen 1; FOS L1; FOS like antigen 1; FOSL 1; FOSL1 protein; FRA 1; Fos-related antigen 1; FOSL1_HUMAN; FRA-1.  
產(chǎn)品類型 磷酸化抗體 
研究領(lǐng)域 轉(zhuǎn)錄調(diào)節(jié)因子  
抗體來(lái)源 Rabbit
克隆類型 Polyclonal
克 隆 號(hào)
交叉反應(yīng) Human,Mouse,Rat (predicted: Rabbit,Pig,Cow,Dog,Horse)
產(chǎn)品應(yīng)用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 28 kDa
檢測(cè)分子量
細(xì)胞定位 細(xì)胞核 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated Synthesised phosphopeptide derived from human FRA1 around the phosphorylation site of Ser265: LG(p-S)PT 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. [provided by RefSeq].

Subunit:
Heterodimer.

Subcellular Location:
Nucleus.

Similarity:
Belongs to the bZIP family. Fos subfamily.
Contains 1 bZIP (basic-leucine zipper) domain.

SWISS:
P15407

Gene ID:
8061

Database links:

Entrez Gene: 8061 Human

Omim: 136515 Human

SwissProt: P15407 Human

Unigene: 283565 Human



FOSL1蛋白對(duì)細(xì)胞因子、生長(zhǎng)因子、感染或致癌刺激等生理或病理信號(hào)發(fā)生應(yīng)答,調(diào)節(jié)基因的轉(zhuǎn)錄, 參與細(xì)胞的增殖、分化等過(guò)程。 FOSL1在腫瘤形成及發(fā)展過(guò)程中, 通過(guò)促進(jìn)細(xì)胞增殖、抑制分化、促進(jìn)腫瘤細(xì)胞的侵襲和轉(zhuǎn)移等過(guò)程發(fā)揮作用。
產(chǎn)品圖片
Sample: Hela(Human) Cell Lysate at 30 ug U87MG(Human) Cell Lysate at 30 ug Primary: Anti- Phospho-FRA1 (Ser265) (bs-3158R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 28 kD Observed band size: 28 kD
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-FRA1 (Ser265)) Polyclonal Antibody, Unconjugated (bs-3158R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Phospho-FRA1(Ser265) Polyclonal Antibody, Unconjugated(bs-3158R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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