| 產(chǎn)品編號 | bs-1560R |
| 英文名稱 | Reelin Rabbit pAb |
| 中文名稱 | 絡(luò)絲蛋白抗體 |
| 別 名 | Reelin; Reeler; RELN; RL; LIS2; PRO1598; RELN_HUMAN. |
| 研究領(lǐng)域 | 細胞生物 免疫學(xué) 神經(jīng)生物學(xué) 細胞凋亡 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應(yīng) | Mouse (predicted: Human,Rat,Pig,Chicken,Dog,GuineaPig,Horse) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 400-450;300;180- kDa |
| 檢測分子量 | |
| 細胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human RELN: 3345-3458/3458 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Reelin (or Reln) is a large glycoprotein that is secreted by Cajal-Retzius cells in the forebrain and by granule neurons in the cerebellum. Reelin was shown to be mutated in “reeler” mice, a mutation that is associated with widespread disruption of laminated regions of the brain, leading to impaired motor coordination, tremors and ataxia. Reelin protein expression is complex and changes throughout development. Reelin appears to function upstream of Dab1 in a signaling pathway that controls cell positioning in the developing brain and is also thought to be a direct effector of the neurotrophin BDNF. Function: This gene encodes a large secreted extracellular matrix protein thought to control cell-cell interactions critical for cell positioning and neuronal migration during brain development. This protein may be involved in schizophrenia, autism, bipolar disorder, major depression and in migration defects associated with temporal lobe epilepsy. Mutations of this gene are associated with autosomal recessive lissencephaly with cerebellar hypoplasia. Two transcript variants encoding distinct isoforms have been identified for this gene. Other transcript variants have been described but their full length nature has not been determined. Subcellular Location: Secreted Tissue Specificity: High level detected in plasma but also in extravascular fluids such as follicular and cerebrospinal fluids (at protein level). Post-translational modifications: N-glycosylated; more than 90% of the glycans are sialylated. Similarity: Belongs to the ALB/AFP/VDB family. Contains 3 albumin domains. SWISS: P78509 Gene ID: 5649 Database links: Entrez Gene: 5649 Human Entrez Gene: 19699 Mouse Omim: 600514 Human SwissProt: P78509 Human SwissProt: Q60841 Mouse Unigene: 655654 Human Unigene: 425236 Mouse Unigene: 98353 Rat Reelin蛋白作用目前還在進一步研究中，但有學(xué)者認為它與β-Amyloid 的神經(jīng)纏結(jié)有相對應(yīng)的作用。 |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Reelin) Polyclonal Antibody, Unconjugated (bs-1560R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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