| 產(chǎn)品編號 | bsm-62534R |
| 英文名稱 | HIF-1 Alpha Recombinant Rabbit mAb |
| 中文名稱 | 缺氧誘導因子1α /HIF-1α重組兔單抗 |
| 別 名 | HIF1; MOP1; PASD8; bHLHe78; HIF-1alpha; HIF1-ALPHA; HIF1A. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | |
| 交叉反應 | Human,Mouse,Rat |
| 產(chǎn)品應用 | WB=1:500-1:2000,ICC/IF=1:50-1:200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 93 kDa |
| 檢測分子量 | 110 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human HIF 1 alpha: 300-550/826 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Functions as a master transcriptional regulator of the adaptive response to hypoxia.Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. SWISS: Q16665 Gene ID: 3091 缺氧誘導因子1α不僅對于機體在缺氧條件下維持正常的生理功能具有特別重要的意義,并在腫瘤的生長以及神經(jīng)細胞凋亡等病理過程中起重要作用. HIF1 alpha能調(diào)節(jié)許多下游基因的表達水平. 哺乳動物細胞在低氧壓力條件下出現(xiàn)HIF。HIF是一種轉(zhuǎn)錄因子，對細胞的缺氧起穩(wěn)定作用。 |
| 產(chǎn)品圖片 |
Western blot analysis of HeLa treated with CoCl2 cell lysate. Using HIF-1 Alpha (bsm-62534R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
