| 產(chǎn)品編號 | bsm-62398R |
| 英文名稱 | gamma Adaptin Recombinant Rabbit mAb |
| 中文名稱 | 銜接蛋白γ/γ1-Adaptin重組兔單抗 |
| 別 名 | ADTG; AP1G1; CLAPG1. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | |
| 交叉反應 | Human,Mouse,Rat |
| 產(chǎn)品應用 | WB=1:1000-1:2000,IHC-P=1:100-1:200,IHC-F=1:100-1:200,IF=1:100-1:200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 91 kDa |
| 檢測分子量 | 100 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human gamma 1 Adaptin: 780-822 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules. SWISS: O43747 Gene ID: 164 γ-Adaptin參與披網(wǎng)格蛋白小泡組裝的一種蛋白質(zhì), 分子量為100kDa, 在披網(wǎng)格蛋白小泡組裝中與受體的細胞質(zhì)結(jié)構(gòu)域相互作用, 起銜接作用。有兩種類型銜接蛋白, AP1參與反面高爾基體的披網(wǎng)格蛋白小泡的組裝, AP2則參與從細胞質(zhì)膜形成的披網(wǎng)格蛋白的組裝。 |
| 產(chǎn)品圖片 |
Western blot analysis of 293T cell lysate. Using gamma Adaptin (bsm-62398R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
