| 產品編號 | bsm-62382R |
| 英文名稱 | HOXA5 Recombinant Rabbit mAb |
| 中文名稱 | 同源異型盒基因HOXA5蛋白重組兔單抗 |
| 別 名 | HOX1; HOX1.3; HOX1C; HOXA5. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | 3D2 |
| 交叉反應 | Human,Mouse,Rat |
| 產品應用 | WB=1:500-1:2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 29 kDa |
| 檢測分子量 | 43 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human HOX A5: 100-150 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Also binds to its own promoter. Binds specifically to the motif 5'-CYYNATTA[TG]Y-3'. SWISS: P20719 Gene ID: 3202 p53基因保護細胞不會癌變，HOXA5協(xié)助細胞的p53保持適當水平。如果HOXA5水平下降或完全缺乏，p53基因就不能防止細胞癌變。低水平的HOXA5通過另一種促甲基化機制使基因不能表達而發(fā)生。正常狀態(tài)下，p53腫瘤抑制蛋白發(fā)現(xiàn)DNA的損傷，將信息傳遞給細胞以修復損傷或者導致死亡。過少或沒有p53不僅減弱細胞正常功能，而且降低細胞感受損傷的能力。因此，若細胞缺少HOXA5蛋白也很可能是腫瘤發(fā)展的重要過程。 |
| 產品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with HOXA5 monoclonal antibody, unconjugated (bsm-62382R) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
