| 產(chǎn)品編號 | bsm-62224R |
| 英文名稱 | MYF5 Recombinant Rabbit mAb |
| 中文名稱 | 生肌決定因子Myf5重組兔單抗 |
| 別 名 | Myf5; Class C basic helix loop helix protein 2. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | 4G8 |
| 交叉反應(yīng) | Mouse,Rat (predicted: Human) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IP=1:20-50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 28 kDa |
| 檢測分子量 | 37 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human MYF5: 201-255/255 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. Together with MYOG and MYOD1, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein. SWISS: P13349 Gene ID: 4617 yf5是MyoD基因家族的一員，該家族的共同結(jié)構(gòu)特點是具有1個保守的中央蛋白基序(motif)，大小為80個氨基酸，稱為堿性螺旋-環(huán)-螺旋(bHLH)，bHLH高度保守，其中Basic結(jié)構(gòu)是HLH螺旋結(jié)構(gòu)的延伸，也是這一家族蛋白與DNA相互作用的區(qū)域，而HLH螺旋結(jié)構(gòu)則是與其他因子相互作用的位點，即調(diào)控的重要區(qū)域。 生肌決定因子(MyoD)基因家族控制著肌細胞的增殖和分化，與肌纖維的數(shù)量、大小有著密切的關(guān)系，因而對肉質(zhì)和風味有非常重要的作用，MyoD基因家族可編碼4種不同的轉(zhuǎn)錄因子，分別為MyoD(Myf |
| 產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with MYF5 monoclonal antibody, unconjugated (bsm-62224R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
