| 產品編號 | bsm-62161R |
| 英文名稱 | NAP1L1 Recombinant Rabbit mAb |
| 中文名稱 | 核小體組裝蛋白1重組兔單抗 |
| 別 名 | NRP; NAP1; NAP1L. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | 8E5 |
| 交叉反應 | Human,Mouse,Rat |
| 產品應用 | WB=1:500-1:2000,Flow-Cyt=1ug/Test,ICC/IF=1:50-1:200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 45 kDa |
| 檢測分子量 | 52,60 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human NAP1L1: 1-55/391 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
May be involved in modulating chromatin formation and contribute to regulation of cell proliferation. SWISS: P55209 Gene ID: 4673 核小體組裝蛋白1（NAP-1） 作為核心組蛋白的分子伴蛋白,在進化過程中是相當保守的.它通過幫助核心組蛋白沉積到DNA上來促進核小體的組裝.而大量研究表明,酵母和動物中的NAP類似蛋白除了具有核小體組裝能力以外,還有其它多種功能 |
| 產品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with NAP1L1 monoclonal antibody, unconjugated (bsm-62161R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (NAP1L1) monoclonal Antibody, unconjugated (bsm-62161R) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-40295G-FITC) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
The Hela (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Rabbit Anti-NAP1L1 antibody (bsm-62161R): 1 μg/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-FITC (bs-40295G-FITC): 1 μg/test. Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
