| 產(chǎn)品編號 | bsm-61922R |
| 英文名稱 | GRIM19 Recombinant Rabbit mAb |
| 中文名稱 | 干擾素/維甲酸誘導凋亡相關(guān)基因重組兔單抗 |
| 別 名 | B16.6; CDA016; CGI-39; GRIM19; GRIM-19. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | |
| 交叉反應 | Human |
| 產(chǎn)品應用 | WB=1:1000-1:2000,IHC-P=1:100-1:200,IHC-F=1:100-1:200,IF=1:50-1:200,ICC/IF=1:50-1:200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 17 kDa |
| 檢測分子量 | 17 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human NDUFA13: 60-100/144 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis.Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. SWISS: Q9P0J0 Gene ID: 51079 GRIM19是GRIMs凋亡相關(guān)基因新家族成員之一,是一種由IFN-β聯(lián)合RA（干擾素/維甲酸）誘導表達的16kDa蛋白,分布在細胞核和胞漿中，GRIM-19是純化的線粒體呼吸鏈復合體I的組成成分,這種復合物為ATP合成所必需的,當線粒體復合物I電子轉(zhuǎn)移產(chǎn)生活性,GRIM-19突變體突破線粒體跨膜電位,可增強細胞對凋亡刺激的敏感性，它的過高表達可以抑制腫瘤細胞的增殖，促進細胞的凋亡。 |
| 產(chǎn)品圖片 |
Western blot analysis of Ramos cell lysate. Using GRIM19 (bsm-61922R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
