| 產(chǎn)品編號 | bsm-61791R |
| 英文名稱 | Calsequestrin Recombinant Rabbit mAb |
| 中文名稱 | 肌鈣集蛋白/收鈣素重組兔單抗 |
| 別 名 | Calmitin; Calmitine; Calsequestrin; Calsequestrin-1; CASQ; Casq1; PDIB1. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | 11G3 |
| 交叉反應 | Human,Mouse,Rat |
| 產(chǎn)品應用 | WB=1:500-1:2000,IHC-P=1:50-1:200,IHC-F=1:50-1:200,IF=1:50-1:200,Flow-Cyt=1:20-1:100
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 45 kDa |
| 檢測分子量 | 63 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human Calsequestrin 1: 100-150/396 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Calsequestrin is a high-capacity, moderate affinity, calcium-binding protein and thus acts as an internal calcium store in muscle. The release of calcium bound to calsequestrin through a calcium release channel triggers muscle contraction. Binds 40 to 50 moles of calcium. Also binds laminin. SWISS: P31415 Gene ID: 844 肌鈣集蛋白，隱鈣素，收鈣素，是肌漿網(wǎng)上調控鈣儲存的主要結合蛋白。CASQ與內質網(wǎng)鈣結合蛋白肌鈣網(wǎng)蛋白具有同源性（calreticulin）。CASQ1主要在快收縮骨骼肌表達，CASQ2主要在在慢收縮骨骼肌和心肌表達。 |
| 產(chǎn)品圖片 |
Western blot analysis of Human skeletal muscle lysate. Using Calsequestrin (bsm-61791R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Calsequestrin Monoclonal Antibody, Unconjugated (bsm-61791R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Calsequestrin Monoclonal Antibody, Unconjugated (bsm-61791R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Calsequestrin Monoclonal Antibody, Unconjugated (bsm-61791R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
