| 產品編號 | bsm-61781R |
| 英文名稱 | Bub1 Recombinant Rabbit mAb |
| 中文名稱 | 紡錘體檢查點蛋白重組兔單抗 |
| 別 名 | Bub1; BUB1A; BUB1L; hBUB1. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | |
| 交叉反應 | Human,Mouse,Rat |
| 產品應用 | WB=1:1000-1:2000,IHC-P=1:100-1:200,IHC-F=1:100-1:200,IF=1:100-1:200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 122 kDa |
| 檢測分子量 | 122 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human BUB1: 1-200/1085 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Serine/threonine-protein kinase that performs 2 crucial functions during mitosis: it is essential for spindle-assembly checkpoint signaling and for correct chromosome alignment. Has a key role in the assembly of checkpoint proteins at the kinetochore, being required for the subsequent localization of CENPF, BUB1B, CENPE and MAD2L1. SWISS: O43683 Gene ID: 699 紡錘體檢查點（spindle checkpoint)是細胞周期中的一個重要檢查點，由Mad2、Bub1等染色體動粒(kinetochore)蛋白構成，像“傳感器”一樣能感知微管與動粒結合情況和張力。紡錘體檢查點阻滯細胞進入分裂后期直到所有染色體著絲點正確與微管聯(lián)接,確保染色體均等分配,維持基因組穩(wěn)定性。 |
| 產品圖片 |
Western blot analysis of K562 cell lysate. Using Bub1 (bsm-61781R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
