| 產品編號 | bsm-61640R |
| 英文名稱 | RNA polymerase beta Recombinant Rabbit mAb |
| 中文名稱 | RNA聚合酶β重組兔單抗 |
| 別 名 | groN; nitB; rif; RNA pol beta; RNA polymerase subunit beta; ron; rpoB; stl; stv; tabD; Transcriptase subunit beta. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | 6H1 |
| 交叉反應 | Escherichia Coli |
| 產品應用 | WB=1:500-2000,IP=1:20-50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 151 kDa |
| 檢測分子量 | 150 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from RNA polymerase beta: 1300-1342/1342 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Resistance to the antibiotics salinamide A, salinamide B, rifampicin, streptolydigin, CBR703, myxopyronin, and lipiarmycin can result from mutations in this protein. |
| 產品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with RNA polymerase beta monoclonal antibody, unconjugated (bsm-61640R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
