| 產(chǎn)品編號 | bsm-61626R |
| 英文名稱 | FSCN1 Recombinant Rabbit mAb |
| 中文名稱 | 纖維束蛋白同源物1重組兔單抗 |
| 別 名 | 55 kDa actin bundling protein; Actin bundling protein; FAN1; Fascin 1; Fascin; Singed(Drosophila) like(sea urchin fascin homolog like); Fascin homolog 1; Fascin. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | 4F8 |
| 交叉反應(yīng) | Human,Mouse,Rat |
| 產(chǎn)品應(yīng)用 | WB=1:1000-1:2000,IHC-P=1:100-1:200,IHC-F=1:100-1:200,IF=1:50-1:200,Flow-Cyt=1:50-100,ICC/IF=1:50-1:200,IP=1:20-1:50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 55 kDa |
| 檢測分子量 | 54 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human Fascin: 200-400/493 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Actin-binding protein that contains 2 major actin binding sites.Organizes filamentous actin into parallel bundles.Plays a role in the organization of actin filament bundles and the formation of microspikes, membrane ruffles, and stress fibers. SWISS: Q16658 Gene ID: 6624 肌動蛋白集束蛋白/圓線蟲紫癜抗體。 FSCN1為細胞骨架肌動蛋白的一種，該蛋白有蛋白結(jié)合,橋連、肌動蛋白絲結(jié)合的功能。 主要參與細胞增殖、肌動蛋白細胞骨架組織和生物發(fā)生、肌動蛋白絲束形成。 |
| 產(chǎn)品圖片 |
Western blot analysis of (1) K562 cell lysate; (2) Mouse kidney lysate. Using FSCN1 (bsm-61626R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
4% Paraformaldehyde-fixed HepG2 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (FSCN1) monoclonal Antibody, unconjugated (bsm-61626R) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-40295G-FITC) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
The HepG2 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Rabbit Anti-FSCN1 antibody (bsm-61626R,1:100); Secondary Antibody (white blue): Goat anti-Rabbit IgG-BF488 (bs-60295G-BF488): 1 μg/test. Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
