| 產(chǎn)品編號(hào) | bsm-61585R |
| 英文名稱 | IDH2 Recombinant Rabbit mAb |
| 中文名稱 | 線粒體異檸檬酸脫氫酶重組兔單抗 |
| 別 名 | D2HGA2; ICD-M; IDH; IDH2; IDHM; IDP; IDPM; mNADP-IDH; NADP(+)-specific ICDH; Oxalosuccinate decarboxylase. |
| 抗體來(lái)源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號(hào) | 2A6 |
| 交叉反應(yīng) | Human,Mouse,Rat |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:50-200,IHC-F=1:50-200,IF=1:50-200,Flow-Cyt=1μg/Test,ICC/IF=1:50-1:200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 51 kDa |
| 檢測(cè)分子量 | 45 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human IDH2: 400-452 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Plays a role in intermediary metabolism and energy production. It may tightly associate or interact with the pyruvate dehydrogenase complex. SWISS: P48735 Gene ID: 3418 IDH2又稱異檸檬酸脫氫酶，他有有兩種類型的酶：以NAD為輔酶的酶（EC1．1．1．41）和NADP的酶（EC1．1．1．42），兩者催化同一反應(yīng)。在胞漿中以NADP為輔酶，在線粒體內(nèi)膜以NAD為輔酶。 IDH2在三羧酸循環(huán)中正常起作用的是NAD為輔酶的酶，他是提供為ADP激活的變構(gòu)酶。 |
| 產(chǎn)品圖片 |
Western blot analysis of MOLT4 cell lysate. Using IDH2 (bsm-61585R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
4% Paraformaldehyde-fixed MCF-7 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (IDH2) monoclonal Antibody, unconjugated (bsm-61585R) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-40295G-FITC) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
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