| 產(chǎn)品編號 | bsm-61534R |
| 英文名稱 | PDCD4 Recombinant Rabbit mAb |
| 中文名稱 | 凋亡相關(guān)蛋白4重組兔單抗 |
| 別 名 | Death up-regulated gene protein; Dug; H731; Ma3; Neoplastic transformation inhibitor; Nuclear antigen H731; Pdcd4; Programmed cell death protein 4; Tis. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | 17B8 |
| 交叉反應 | Human,Mouse |
| 產(chǎn)品應用 | WB=1:500-2000,IHC-P=1:50-200,IHC-F=1:50-200,IF=1:50-200,Flow-Cyt=1:20-50,ICC/IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 52 kDa |
| 檢測分子量 | 52 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human PDCD4: 400-469 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Inhibits translation initiation and cap-dependent translation. May excert its function by hindering the interaction between EIF4A1 and EIF4G. Inhibits the helicase activity of EIF4A. Modulates the activation of JUN kinase. Down-regulates the expression of MAP4K1, thus inhibiting events important in driving invasion, namely, MAPK85 activation and consequent JUN-dependent transcription. SWISS: Q53EL6 Gene ID: 27250 程序性細胞死亡因子4是近期發(fā)現(xiàn)一種新的抑癌基因，在很多腫瘤組織中有不同的表達。 |
| 產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with PDCD4 monoclonal antibody, unconjugated (bsm-61534R) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
