| 產(chǎn)品編號(hào) | bsm-61405R |
| 英文名稱 | Parvalbumin Recombinant Rabbit mAb |
| 中文名稱 | 細(xì)小清蛋白重組兔單抗 |
| 別 名 | Parvalbumin alpha; PVALB. |
| 抗體來(lái)源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號(hào) | 11H20 |
| 交叉反應(yīng) | Human,Mouse,Rat |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,IP=1:20-50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 12 kDa |
| 檢測(cè)分子量 | 12 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human Parvalbumin alpha: 1-110/110 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
In muscle, parvalbumin is thought to be involved in relaxation after contraction. It binds two calcium ions. SWISS: P20472 Gene ID: 5816 (Parvalbumin, PV)細(xì)小白蛋白屬于鈣結(jié)合蛋白家族,是一種細(xì)胞內(nèi)可溶的小結(jié)合蛋白，過(guò)去曾認(rèn)為，僅出現(xiàn)在哺乳動(dòng)物某些肌纖維內(nèi),它與Ca2+有高度親合力,與鈣調(diào)蛋白競(jìng)爭(zhēng)自由Ca2+,故可阻止肌漿內(nèi)的Ca2+增高，可抗衡細(xì)胞內(nèi)鈣過(guò)量的發(fā)生。 |
| 產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with Parvalbumin monoclonal antibody, unconjugated (bsm-61405R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Parvalbumin Monoclonal Antibody, Unconjugated (bsm-61405R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Parvalbumin Monoclonal Antibody, Unconjugated (bsm-61405R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Parvalbumin Monoclonal Antibody, Unconjugated (bsm-61405R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Parvalbumin Monoclonal Antibody, Unconjugated(bsm-61405R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Parvalbumin Monoclonal Antibody, Unconjugated(bsm-61405R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Parvalbumin Monoclonal Antibody, Unconjugated(bsm-61405R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Parvalbumin Monoclonal Antibody, Unconjugated (bsm-61405R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Parvalbumin Monoclonal Antibody, Unconjugated(bsm-61405R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
(Negative control)Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Parvalbumin Monoclonal Antibody, Unconjugated(bsm-61405R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
|
