| 產(chǎn)品編號 | bsm-61370R |
| 英文名稱 | RIPK1 Recombinant Rabbit mAb |
| 中文名稱 | 絲氨酸蘇氨酸激酶1受體相互作用蛋白重組兔單抗 |
| 別 名 | RIPK1; Cell death protein RIP; RIP1; RIP; RIP-1; Rinp. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | WB=1:1000-1:2000,Flow-Cyt=1:20-1:100
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 76 kDa |
| 檢測分子量 | 75 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human RIP 1: 289-490 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Essential adapter molecule for the activation of NF-kappa-B. Following different upstream signals (binding of inflammatory cytokines, stimulation of pathogen recognition receptors, or DNA damage), particular RIPK1-containing complexes are formed, initiating a limited number of cellular responses. SWISS: Q13546 Gene ID: 8737 受體相互作用蛋白1是一類絲氨酸/蘇氨酸蛋白激酶，是一種重要的細胞信號轉(zhuǎn)導(dǎo)分子，他不僅參與了細胞的凋亡，還參與了細胞存活，細胞程序性壞死等多種信號的轉(zhuǎn)導(dǎo)，是一類重要的調(diào)節(jié)細胞生存或死亡的信號分子，通過激活NF-κB、活化caspase-8、參與活性氧的產(chǎn)生等,在細胞凋亡、細胞存活及細胞程序性壞死等信號傳導(dǎo)中起關(guān)鍵作用,其功能受泛素化、鋅指蛋白及熱休克蛋白等的調(diào)節(jié)。 |
| 產(chǎn)品圖片 |
Western blot analysis of HeLa cell lysate. Using RIPK1 (bsm-61370R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
