| 產(chǎn)品編號 | bsm-61349R |
| 英文名稱 | Phospho-CDC42 (Ser71) Recombinant Rabbit mAb |
| 中文名稱 | 磷酸化細胞分化周期CDC42蛋白重組兔單抗 |
| 別 名 | CDC42; CDC42Hs; G25K; TKS; MIG5; Ras like protein TC25. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | |
| 交叉反應 | Human |
| 產(chǎn)品應用 | WB=1:1000-1:2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 21 kDa |
| 檢測分子量 | 24 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human Cdc42 around the phosphorylation site of S71: PL-pS-YP |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Rac and Cdc42 are members of the Rho-GTPase family. In mammals, Rac exists as three isoforms, Rac1, Rac2 and Rac3, which are highly similar in sequence. Rac1 and Cdc42, the most widely studied of this group, are ubiquitously expressed. Rac2 is expressed in cells of hematopoietic origin, and Rac3, while highly expressed in brain, is also found in many other tissues. Rac and Cdc42 play key signaling roles in cytoskeletal reorganization, membrane trafficking, transcriptional regulation, cell growth and development. SWISS: P60953 Gene ID: 998 Cdc42，是細胞骨架和細胞極化的重要調節(jié)蛋白，在減數(shù)分裂和卵母細胞成熟過程中有重要的作用，尤其在細胞分化，遷移過程中起到一定的作用,目前在腫瘤研究中得到了重視。 |
| 產(chǎn)品圖片 |
Western blot analysis of (1) A431 cell lysate treated with LP; (2) A431 cell lysate. Using Phospho-CDC42 (Ser71) (bsm-61349R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
